Search NORF data

Our transposon-tagging approach is an effective means of identifying previously non-annotated open reading frames (NORFs) within the yeast genome. We have gathered together insertion point data for all strains carrying a transposon insertion within a putative NORF; this dataset may be studied by performing a category-specific search of NORF data. To illustrate, we may search this dataset to identify any potential NORFs in the vicinity of NPL3, a gene involved in the processing of 18S and 25S rRNA. By entering "NPL3" in the "genome region" field, we see a table listing insertion point data for strains carrying a transposon insertion in a NORF near NPL3. We may subsequently obtain a full composite report for any indicated strain within this table (e.g., V9E11, V56F3); these composite reports allow us to identify any disruption phenotypes and/or subcellular localization associated with this mTn-tagged gene. Note the V56F3 strain exhibits growth defects on YPGlycerol as well as hypersensitivity to cycloheximide and hygromycin.

To carry out the search described above, please select option 1 below. Alternatively, you may select option 2 to access our "NORF data" search form to perform a search of your own.