GENERAL SEARCHES

Our database can be searched in two ways:

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GENERAL SEARCH QUERY RESULTS

Search results are formatted as a table with each row containing information for a given strain carrying the indicated transposon insertion.

 

 

 

 

 

 

 

 

 

 

 

 

 

Potential ORFs Disrupted by mTn Insertion

A single transposon insertion event may disrupt any of a number of open reading frames (ORFs) within a given region of the yeast genome. For example, a nonannotated ORF (NORF) may overlap a previously annotated ORF; a transposon insertion within this overlapping region may, therefore, disrupt either the NORF or annotated ORF. In this case, both the NORF and annotated ORF would be listed in our table.

 

 

 

 

 

 

 

 

 

 

 

 

 

Insertion Point Data

A variety of underlying mTn insertion events are responsible for b-gal activity within the strains constituting our collection. We have classified these insertion events as follows:

 

 

 

 

 

 

 

 

 

 

 

 

 

Gene Expression Data

To determine whether a given transposon-tagged gene is expressed during vegetative growth or sporulation, transposon-mutagenized yeast genomic DNA is transformed into the indicated background strain; strains are then grown on rich medium and sporulation medium for approximately 17-20 hours. b-gal activity is subsequently measured using a filter-based assay.

LacZ expression levels are determined from the observed intensity of blue staining after growth on medium containing X-gal. Stain intensity is scored as follows:

 

 

 

 

 

 

 

 

 

 

 

 

 

Subcellular Localization

Strains carrying a HAT-tagged protein were examined by indirect immunofluorescence using monoclonal antibodies directed against the transposon-encoded HA epitope.

Fluorescent micrographs are available for any highlighted localizations. Please click on the underlined localization entry to view a corresponding IF image of yeast cells stained with the DNA-binding dye DAPI (left-hand image) and the same cells stained by indirect immunofluorescence using Anti-HA antibodies (right-hand image).

Please bear with our data — we endeavor to classify observed staining patterns as thoroughly as possible

Disruption Phenotype Data

Haploid strains (each carrying an independent mTn insertion) were screened for disruption phenotypes using the following assays (growth conditions):

Background strain and its ploidy are also included per entry:

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Search NORF Data

In annotating the S. cerevisiae genome, an arbitrary lower size limit of 100 amino acids was adopted as convention in the absence of specific information to the contrary. Therefore, typically, open reading frames <100 codons in length are not annotated. These non-annotated ORFs (NORFs), however, are capable of being translated; some may be of significant biological relevance. Our transposon-tagging approach is an effective means of identifying NORFs -- we have collected data concerning these NORFs for easy access using this search form.

NORFs are sometimes found overlapping (or within) larger annotated ORFs; to specifically search for a NORF located within (or in the vicinity of) a given gene, please enter that gene name within the "genome region" field.

For an explanation of indicated categories, please read our insertion point data help text.