Welcome to TRIPLES — a database of TRansposon-Insertion Phenotypes, Localization, and Expression in Saccharomyces.

We have developed this database as a means of providing complete public access to the data generated from our transposon-tagging study of the yeast genome. As described on our home page, we have developed a transposon-based approach for the large-scale analysis of gene function. From a single transposon insertion event, we can generate three types of data concerning gene function:

	Using transposon-encoded lacZ as a reporter, we have performed b-gal filter assays to determine when a given gene is expressed during the yeast life cycle.
	Transposon insertion results in truncation of the host gene, enabling us to determine disruption phenotypes on a large-scale by macroarray analysis.
	The subcellular localization of transposon-tagged proteins has been analyzed using monoclonal antibodies directed against the mTn-encoded HA epitope.

All three classes of data may be freely accessed using our search form

TRIPLES Demo